Rab5 activity
pRaichu-Rab5, derived from the pCAGGS expression vector, encoded a probe, designated Raichu-Rab5, that comprised Venus (the brightest version of YFP)21, the amino-terminal Rab5-binding domain of EEA1 (amino-acid residues 36–218), SECFP (a brighter version of CFP obtained from A. Miyawaki) and Rab5a … See more C57BL/6 mice were purchased from Japan SLC. EGFP-transgenic mice19 were a gift from M. Okabe. Swiss3T3 fibroblasts stably expressing … See more Swiss3T3/integrin αvβ3cells were transfected with the desired siRNAs by using Lipofectamine RNAiMAX (Invitrogen). siRNA sequences for control, mouse Rabex-5, mouse Rin1 and mouse Gapex-5 were 5′ … See more Apoptotic thymocytes were prepared as described previously4. In brief, thymocytes of 4–8-week-old C57BL/6 mice or EGFP-transgenic mice were … See more Swiss3T3/integrin αvβ3 cells expressing FRET probes were cultured in phenol-red-free DMEM/F12 medium supplemented with 10% fetal bovine serum, and loaded with 4 × 106 apoptotic thymocytes in the presence of 0.1 μg ml … See more Web• Role of LRRK2 kinase and luminal acidification in the regulation of Rab5 activity in endosomes via Rab5 GAPs. • Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK. • Role of NCOA7b in the …
Rab5 activity
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WebMar 12, 2024 · The Rab5 activation network of Rab5:GDI, Rabex5:Rabaptin5, and the membrane is intrinsically stochastic and bistable. The switching probability depends on the amount of membrane-bound Rab5:GDP, which in turn is controlled by GDI extraction and the presence of a GDF such as PRA1 that counters the GDI activity. WebI was born in Alexandroupolis in 1993, a city located by the coast of the northeastern part of Greece. During my childhood, curiosity got the best of me about almost anything. My growing interest in science began shortly after finishing elementary school. Because of personal experiences and beliefs, I was strongly motivated to start a professional career …
WebMay 13, 2014 · This work identifies the Arabidopsis MON1 and CCZ1 proteins as forming a dimeric complex that functions as a Rab5 effector and a Rab7 GEF to mediate Rab5-to-Rab. Skip to ... (Rab7) in a dose-dependent manner, while this complex showed limited GEF activity for RHA1 (Rab5). In addition, in protoplasts, this MON1-CCZ1 complex ... WebApr 18, 2024 · The activity of Rab5 was assessed by a Rab5 activation assay kit (83701; NewEast Biosciences, King of Prussia, PA), according to the manufacturer’s recommended protocol. In brief, cell lysates containing equal amounts of proteins in equal volumes were incubated with a mouse monoclonal antibody specifically recognizing GTP-bound Rab5, …
WebMore recently, glutathione S-transferase (GST) pull-down assays [ 6] have been adopted to determine Rab5 activity in the cell (Fig. 1 ). In this case, GST fusion proteins with a Rab5 effector, which specifically binds to GTP-bound Rab5, is bound to glutathione-coupled Sepharose beads and used to affinity-purify Rab5-GTP in cell lysates. WebMay 11, 2024 · The existence of two RAB5 groups (i.e. canonical and plant-unique) is a distinctive feature of plant cells. In 2011, researchers showed that a plant-unique RAB5 could interfere with and counteract the activities of a canonical RAB5. However, it remained ambiguous how these proteins could do this.
WebIn any case, the observation that Rab5 activity promotes internalization and decreases G q inhibitory function of RGS4 is a novel observation that will likely inform the design of new experiments to test whether activators and inhibitors of Rab5 function may be used to modulate RGS4 expression and function at the plasma membrane.
WebMoreover, longevity of animals lacking bulli is affected. Both the Mon1–Ccz1 dimer and a Bulli-containing trimer display Rab7 GEF activity. In summary, this suggests a key role for Bulli in the Rab5 to Rab7 transition during endosomal maturation rather than a direct influence on the GEF activity of Mon1–Ccz1. Show less umast membershipWebAug 29, 2024 · The endocytic pathway is a system of dynamically communicating vesicles, known as early endosomes, that internalize, sort, and traffic nutrients, trophic factors, and … umass work studyWebAug 15, 2007 · Rabex-5 is a guanine nucleotide exchange factor (GEF) for Rab5. Here, we report the identification of a novel functional domain of Rabex-5 that is essential for its membrane targeting and Rab5 GEF activity in vivo. The data show that full-length Rabex-5 efficiently activates Rab5 in the cell. However, the GEF domain itself (residues 135–399) … uma suthersanenWebRab5 is a conserved master regulator of membrane trafficking; it regulates the formation of early endosomes as well as their transport along microtubules and fusion to the target organelles. I identified Rab5 GTPase in a phenotypic screen for small GTPases involved during mitosis. In this thesis, I describe a novel role for Rab5 in cell division in … thorium mod 1.4 portWebJul 2, 2024 · Further, since the alpha isoform of p38 mitogen-activated protein kinase (p38α) is a major regulator of Rab5 activity and its effectors, a biology that is distinct from the classical nuclear targets of p38 signaling, brain-penetrant selective p38α kinase inhibitors provide the opportunity for significant therapeutic advances in neurogenerative disease … umass worcester weight loss clinicWebFirst, HBV requires Rab5- and Rab7-dependent transport through the endosomal compartment to initiate a successful infection. 38 On the other hand, HBV can suppress autophagosome maturation and escape autophagic degradation via the negative regulation of Rab7 expression. 39 In addition, Rab33B is necessary for the reproduction of HBV and … umas webpartsWebA key regulator of receptor-mediated endocytosis, Rab5, plays a pivotal role in cargo receptor internalization, endosomal maturation, and transduction and degradation of … umass wrestling