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Psh65 plasmid

WebOct 25, 2024 · To obtain the engineered Saccharomyces cerevisiae for high-yield endoglucanase, endoglucanase gene was cloned from Clostridium cellulovorans, with a total length of 1 996 bp, encoding 440 amino... WebThe present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene …

pSH65 (P30122) - Euroscarf

WebpSH65: Cre-loxP plasmid used to remove the kanMX gene from the LycoYeast strain. Encodes for the Cre-recombinase under the control of the galactose-inducible promoter … WebSelection Ampicillin, delta-ura3 Source Yeast, Escherichia coli Species Bacterial Q&A Customer Reviews Related Products For research use only. Not intended for any clinical use. Quick Inquiry Name Service & Products Interested * pSH65 (OVT2571) RESET USA SUITE 115, 17 Ramsey Road, Shirley, NY 11967, USA Germany Industriepark Höchst, Gebäude … how to create datasets for machine learning https://goodnessmaker.com

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WebThe Kan (r) marker was rescued by transforming plasmid pSH65 into positive transformants and inducing expression of Cre recombinase in galactose-containing medium. Lastly, the … WebUse pSH65 for functional genomics research and drug discovery study. 2024-nCoV Research Solution Guide; Monkeypox Virus Real Time PCR Kit; ... Non-GMP Plasmid … WebThe above-mentioned integrated plasmid contains components: pMB1 replicon, ampicillin resistance encoding gene, G418 resistance selection marker KanMX, δ1 fragment, δ2 fragment, GPD promoter, ADH1 terminator, TEF1 promoter and CYC1 terminator. The integrated plasmid is referred to as plasmid pND04 for short. microsoft rewards 999 day streak

The molecular characterization and immune protection of

Category:Addgene: Vector Database - pSH

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Psh65 plasmid

Human cytomegalovirus protein pp65: an efficient protein carrier syste…

WebApr 11, 2016 · The G418-resistant KanMX gene was excised with Cre recombinase and expressed from the pSH65 plasmid (Gueldener et al. 2002 ). Heterogeneous expression of JJJ1 in BYΔJJJ1 was performed by cloning the JJJ1 ORF into the EcoR I and Kpn I sites of the pYES2 plasmid (Invitrogen, Carlsbad, CA, USA). Webthe plasmid pUG6 as a template (Gueldener et al. 2002). Saccharomyces cerevisiae cells were trans- ... containing 300 lg G418/ml. The G418-resistant KanMX gene was excised with Cre recom-binase and expressed from the pSH65 plasmid (Gueldener et al. 2002). Heterogeneous expression of JJJ1 in BYDJJJ1 was performed by cloning the JJJ1 ORF …

Psh65 plasmid

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WebPlasmid Files. Download over 2,700 carefully annotated plasmid and sequence files including commonly used cloning vectors from all major suppliers. Explore Over 2.7k … WebJun 1, 2015 · Then plasmid pSH65, containing a zeocin resistance gene (Bler) was transferred into A. limacinum OUC_CG. After induction with galactose, repeated passage …

WebDec 6, 2007 · Due to the efficient induction of pp65-specific T-cell responses in HCMV-infected individuals, 34, 35, 36 we hypothesized that pp65 itself might, as a soluble … WebThis modified strain was transformed with the pSH65 plasmid ( Gueldener et al., 2002 ), incubated for 5 h in non-selective medium, and plated on solid medium supplemented with phleomycin (400 μg/ml).

WebApr 10, 2012 · To remove the hygromycin B resistance marker gene hphNT1, IMI058 and IMI059 were transformed with pSH65 and—after marker removal via the Cre/loxP system … WebThe highest XynA secretion efficiency level in deep 24-well plates level (2492.88 U/mL) was achieved by disruption of the cell wall protein CspB2 and the protease ClpS, chromosomal integration of...

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microsoft rewards achievement glitchWebMar 15, 2002 · For construction of plasmid pSH65 ( GAL1-cre, ble) the vector pUT332, containing the bler gene from transposon Tn 5 plus the S.cerevisiaeTEF1 promoter ( 34 ), … how to create date column in sqlWebBackground: Adherence to the surface of the host cell is the precondition for T. vaginalis parasitism and pathogenicity, causing urogenital infection. The AP65 of T. vaginalis … how to create datawarehouse tables in synapseWebApr 14, 2024 · In addition, using the electrotransformation to remove the selected marker KanMX [ 32 ], the pSH65 plasmid was transformed into haploid yeast strains with LEU1 and PDC5 deletions, and finally, the recombinant yeast strains XFa7-P/XFα6-P and XFa7-L/XFα6-L were obtained. microsoft rewards account dashboard sign inWebNov 2, 2015 · Here, we present a summary of our recent findings on the (patho-)physiological relevance of PINK1-phosphorylated ubiquitin (p-S65-Ub). Using novel … how to create date field in htmlWebApr 13, 2024 · The aim of the study was to transfer two plasmids, pRS425-HygR-CYP5150L8 and pRS426-KanMx-CYP5139G1, into S. cerevisiae YL-T3 cells to generate DHLDOA-producing strain Y2P and to add hygromycin and G418 to avoid plasmids loss during cell cultivation. In addition, it was necessary to transfer plasmid pRS424-ZeoR-CPR into Y2P … how to create date from period name power biWebPlasmid: pSH. Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse … how to create date in groovy